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NMRI strain mice, between 6 and 10 weeks of age, were supplied by Charles River Laboratories or bred in house at the Bernhard Nocht Institute for Tropical Medicine.

Experiments were conducted in accordance with European regulations and approved by local authorities.

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Hidden Markov Model (HMM) profiles were constructed from each of the MSAs using HMMER3 (Eddy 2009).We do our best to delete links to inappropriate content expeditiously, when it is reported.We will investigate and take action against anyone abusing the DMCA notification or counter-notification procedure.This procedure was repeated for the next 10–12 days, and the sexual stages were harvested at different time points using MACS separation columns (Miltenyi Biotec, Germany).For transfection, ring-stage parasites (10%) were electroporated with 100 μg of plasmid DNA resuspended in cytomix as previously described.

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